Penicillin ELISA Kit is a competitive ELISA kit to detect Penicillins in milk, milk powder, honey and cell culture samples.
Penicillins are a group of antibiotics used to treat a wide range of bacterial infections. They are derived from fungi, and their medical properties were discovered in 1928. Penicillin antibiotics stop bacteria from multiplying by preventing bacteria from forming the walls that surround them. The walls are necessary to protect the bacteria from their environment, and to keep the contents of the bacterial cell together. Bacteria cannot survive without a cell wall. Penicillin antibiotics are most effective when bacteria are actively multiplying and forming cell walls.
This Penicillin ELISA Kit is based on indirect competitive ELISA, the coupled penicillin antigen was pre-coated in the microwells, the penicillin will compete for the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of penicillin concentration in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of penicillin in the sample.
Key facts of the Penicillin ELISA Kit
- Ready to use ELISA kit based on Competitive Immunoassay
- Total time required only 60min
- Highly specific to penicillins.
- Applicable for milk, milk powder, honey and cell culture sample
Penicillin ELISA kit parameters
- Sensitivity: 0.05 ppb, ng/ml
- Stability: 12 months when stored properly
- Accuracy: 70%-120%
Penicillin ELISA kit components
- Precoated microwell, 96wells
- Penicillin standard series, 0.05 - 4.05 ng/ml
- Enzyme conjugate
- TMB Substrate
- Stop solution (not available when shipping by air)
- Sample buffer
- Wash buffer
Further readings on Penicillin and Penicillin testing:
- Gustavsson, E., Bjurling, P., & Sternesjö, Å. (2002). Biosensor analysis of penicillin G in milk based on the inhibition of carboxypeptidase activity. Analytica Chimica Acta, 468(1), 153-159.
- Korycka-Dahl, M., Richardson, T., & Bradley Jr, R. L. (1985). Use of microbial β-lactamase to destroy penicillin added to milk. Journal of dairy science, 68(8), 1910-1916.