Total Aflatoxin ELISA Kit

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Kit Principle
Test Type



Total Aflatoxin ELISA Kit is a competitive ELISA kit to detect contamination of total aflatoxins in cereal such as wheat, corn, beans and sorghum samples.

Aflatoxins are a class of chemical compounds produced by three species of Aspergillus - Aspergillus flavus, Aspergillus parasiticus, and the rare Aspergillus nomius - which contaminate plant and plant products. Aspergillus flavus produces only B-type aflatoxins. Aflatoxins include a series of toxic chemicals, such as B1, B2, G1, G2, M1, M2, etc.

It is known as the most carcinogenic chemical. After intake of aflatoxin contaminated feed in domestic animals, aflatoxins will be taken into human table. Thus, detection and elimination of aflatoxins in feed and cereals are very important. Now strict residue limit of Aflatoxins are established in EU, USA, China, etc. 

This ELISA Kit is based on indirect competitive ELISA, the coupled antigen was pre-coated in the microwells, the aflatoxins will compete the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of aflatoxins contained in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of aflatoxins in the sample.

Key facts of the Total Aflatoxin ELISA Kit

  • Ready to use ELISA kit based on Competitive Immunoassay
  • Total time required only 45min
  • Highly specific to aflatoxin B1, B2, G1 and G2. 
  • Applicable for cereals (including wheat, corn, beans and sorghum, etc)

Total Aflatoxin ELISA Kit parameters

  • Sensitivity: 0.01 ng/ml (ppb)
  • Stability: 12 months when stored properly
  • Accuracy: 80%-120%

Total Aflatoxin ELISA Kit components

  • Precoated microwell, 96wells
  • aflatoxin standard series, 0.10 - 8.10 ng/ml
  • Enzyme conjugate
  • Antibody solution
  • TMB Substrate
  • Stop solution (not available when shipping by air)
  • Sample buffer
  • Wash buffer

Total Aflatoxin ELISA Kit related products

For more information about the aflatoxin B1 ELISA kit, please feel free to contact us.

Further readings on aflatoxins and aflatoxin testing: