Nonylphenol ethoxylates, NPE ELISA Kit

Product Code
Kit Principle



Nonylphenol ethoxylates, NPE ELISA Kit is a competitive ELISA kit to detect NPE in milk and milk powder samples. Water samples may also apply.

NPEs are nonionic surfactants that are used in a wide variety of industrial applications and consumer products. Many of these, such as laundry detergents, are “down-the-drain” applications. Some others, such as dust-control agents and deicers, lead to direct release to the environment. NPEs, though less toxic and persistent than NP, are also highly toxic to aquatic organisms, and, in the environment, degrade into NP.

Nonylphenol (NP) and nonylphenol ethoxylates (NPEs) are produced in large volumes, with uses that lead to widespread release to the aquatic environment. NP is persistent in the aquatic environment, moderately bioaccumulative, and extremely toxic to aquatic organisms. NP has also been shown to exhibit estrogenic properties in in-vitro and in-vivo assays. NP’s main use is in the manufacture of NPEs. NP and NPEs have been found in environmental samples taken from freshwater, saltwater, groundwater, sediment, soil, and aquatic biota. NP has also been detected in human breast milk, blood, and urine and is associated with reproductive and developmental effects in rodents.

This NPE ELISA Kit is based on indirect competitive ELISA, the coupled antigen was pre-coated in the microwells, the NPE will compete for the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of NPE contained in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of NPE in the sample.

Key facts of the NPE ELISA Kit

  • Ready to use ELISA kit based on Competitive Immunoassay
  • Total time required only 75min
  • Highly specific to NPEs, including NPE-4, NPE-7, NPE-10, etc.
  • Applicable for milk sample

NPE ELISA Kit parameters

  • Sensitivity: 5 ppb, ng/ml
  • Stability: 12 months when stored properly
  • Accuracy: 80%-120%

NPE ELISA Kit components

  • Precoated microwell, 96wells
  • nonylphenol polyoxyethylene ether NPE standard series, 0.02 - 1.62 ng/ml
  • Enzyme conjugate
  • TMB Substrate
  • Stop solution (not available when shipping by air)
  • Sample buffer
  • Wash buffer

For more information about the NPE ELISA kit, please feel free to contact us.

Further readings on NPEs and NPEs testing