Babesia Gibsoni Real-time PCR Test Kit

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Kit Principle
Test Type



Babesia Gibsoni Real-time PCR Test Kit is to detect Babesia gibsoni nucleic acid in canine and feline blood sample, which is rapid, accurate and easy-to-operate. Sensitivity 100%, Specificity 100%.

Babesia gibsoni is a tickborne protozoal blood parasite that causes hemolytic anemia, thrombocytopenia, lethargy, and splenomegaly. In the United States, the disease primarily affects American pit bull terriers and is often subclinical. Other breeds of dogs are less often infected and may have a history of fighting with a pit bull terrier. Diagnosis is most often made by identifying the organism on blood smears or through polymerase chain reaction testing. Serologic assays are less sensitive and specific. In many cases, treatment is frustrating, low levels of parasites remain, and a chronic subclinical carrier state develops.

This kit uses fluorescence probe PCR (RT-PCR) method to detect the deserved gene of Babesia gibsoni, which is helpful for diagnosis, monitoring and epidemiological investigation of Babesia gibsoni

Key facts of the Babesia Gibsoni Real-time PCR Test Kit

  • Ready to use kits for the vet clinic
  • No extraction required
  • Result in 30min.

Babesia Gibsoni Real-time PCR Test Kit Components

Item # Item Qty
1 PCR reaction solution 120ul
2 Negative Control 50ul
3 Positive Control 50ul
4 Exogenous Gene Control 50ul
5 Sample buffer 1ml
6 Enzyme mix 10ul
7 Kit user manual 1set

What is babesiosis?

Babesia infection or babesiosis refers to a tick-borne infection due to a protozoal parasite. Babesia invades mammalian red blood cells causing anemia. Babesia species are found worldwide, although in North America, most canine cases of babesiosis occur in the southern United States. Babesiosis is considered a serious threat to racing greyhounds and pit bull terriers.

How do cats and dogs get babesiosis?

Babesiosis is spread primarily through the bite of an infected tick (various species of ticks can carry the disease). There is also evidence that some direct animal-to-animal transmission may occur, for example when an infected dog with an oral lesion or abrasion bites another dog. This is especially true for the Babesian Gibson, which primarily affects Bull Terriers. Recent research suggests that Babesia may be transmitted through the placenta. Dogs can also become inadvertently infected through contaminated blood transfusions. Dogs kept in kennel environments with poor tick control are at higher risk for babesiosis. Cats are most commonly infected with babesiosis through tick bites. Babesiosis has been found in a variety of vertebrates, including humans. Fortunately, it's not a zoonotic disease, which means you can't catch it from a cat. Because it is a blood-borne parasite, it can be transmitted to other cats through bites, scratches, or even blood transfusions.

What are the clinical symptoms of cats and dogs infected with Babesia?

The most consistent findings on physical examination were mucosal pallor with varying degrees of fever, hepatosplenomegaly, jaundice, dehydration, and shock. Severe intravascular hemolysis associated with virulent babesiosis results in hemoglobinuria (“red water”), and affected dogs develop symptoms reflecting widespread organ dysfunction associated with hypotension, hypoxemia, and extensive tissue damage Signs such as anuria or oliguria, neurological deficits, coagulopathy, and acute respiratory distress. Petechiae and petechial bleeding may be observed on the gums or ventral abdomen in some dogs, which is consistent with concurrent thrombocytopenia or thrombocytopathy There is agreement. Dogs that survive the initial infection become lifelong carriers of the parasite despite proper treatment and resolution of the initial signs. Many dogs remain subclinical, although intermittent parasitemia and re-entry of parasites into the blood may occur after immunosuppressive therapy or concurrent disease. Feline babesiosis tends to present as an afebrile, chronic, low-grade disease. In one study, anorexia, depression, and pallor were the most common clinical signs of feline babesiosis, with less weight loss, jaundice, constipation, and pica recorded.

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