Bluetongue virus BTV Antibody Indirect ELISA Kit
Bluetongue virus (BTV) is the pathogen responsible for bluetongue disease. This disease, transmitted mainly by Culicoides midges and infecting cattle, sheep, goats and other ruminants, often presents with oral mucosal erosion, tongue cyanosis, coronitis and reproductive disorders. It is a highly contagious OIE-notifiable animal disease and a critical barrier to global livestock trade, inflicting massive economic losses on the breeding industry through abortion, reduced production performance and mandatory quarantine restrictions.
In bluetongue disease (BT) diagnostics, the VP7 protein is the major, highly conserved core structural protein of the virus. Because its sequence is relatively stable across all serotypes, it serves as the primary target for group-specific sero-diagnostics (detecting exposure to any bluetongue virus strain) rather than identifying a specific serotype.
This Bluetongue virus BTV Antibody Indirect ELISA Kit is based on indirect ELISA to detect BTV VP7 protein specific antibodies in bovine serum.
Key facts of this Bluetongue virus BTV Antibody Indirect ELISA Kit
- Ready to use kits for veterinary laboratories
- Applicable for large quantity testing
- Less than 120min to get result
- Low cost with high sensitivity
Technical Information for the Bluetongue virus BTV Antibody Indirect ELISA Kit
| Product Code | E40162 |
|---|---|
| Other names | BTV Antibody ELISA Kit Bluetongue Antibody ELISA Kit |
| Package Size | 1*96wells, 2*96wells, 5*96wells, 10*96wells, |
| Shelf-life | 12 months |
| Storage | 2-8 degree Celsius at cool dark place |
| Delivery | Room temperature |
| Sensitivity | >95% |
| Specificity | >99% |
| Application | This kit can be used for the detection of bluetongue virus VP7 protein specific antibodies in bovine serum. |
| Reproducibility | Inter-lot C.V. <10%; Intra-lot C.V. <5% |
| Total time | 75min |
| ELISA reading | 450nm with ELISA reader |
| Approval status | Pending approval |
Bluetongue virus BTV Antibody Indirect ELISA Kit Components
- Positive & Negative Control
- Microplate pre-coated with recombinant VP7 protein
- HRP enzyme conjugate
- TMB Substrate
- Stop solution
- Sample buffer
- Wash buffer
Please notice that the kit component above is only for information purpose. You should also check the kit content for details.
Performance of the Bluetongue virus BTV Antibody Indirect ELISA Kit
The Bluetongue virus BTV Antibody Indirect ELISA Kit is used to measure the quantity of antibodies by wild infection. In the field trial with 615 serum samples shows that this kit has a specificity of 99.2%.
Extended Reading
- Biswas, S. K., Hosamani, M., Chand, K., Chauhan, A., Ain, K. U., Selvarajan, V., Nautiyal, S., Bashir, M., Hemadri, D., Sharma, G. K., & Sreenivasa, B. P. (2025). Expression of bluetongue virus full-length VP7 protein in insect cells and its diagnostic utility for detection of antibodies to the virus infection. Journal of immunological methods, 538, 113801. https://doi.org/10.1016/j.jim.2025.113801
- Hu, XB., Xu, J., Zhang, Y. et al. Monoclonal antibody and B-cell epitope mapping of the VP7 protein in bluetongue virus. Virol J 22, 129 (2025). https://doi.org/10.1186/s12985-025-02733-7
- Gandhale, P. N., Bhanuprakash, V., Balamurugan, V., Hosamani, M., Venkatesan, G., & Singh, R. K. (2010). Detection of bluetongue virus group-specific antigen using monoclonal antibody based sandwich ELISA. Virologica Sinica, 25(6), 390–400. https://doi.org/10.1007/s12250-010-3160-y
Frequently asked questions
Unlike the other manufacturer of research reagents, our products are well validated according to ISO standards and are designed for diagnostic purpose. Some of them are also validated by International Institute, such as AOAC-RI, ILVO, WOAH reference laboratories. Using our products, you will have reliability, affordability, and credibility. Our technical support team will also provide after-sale service in case you have any technical questions.
