Melamine ELISA Kit

Melamine ELISA Kit is a competitive ELISA kit to detect melamine in milk, milk powder, and feed sample.

Melamine is a chemical compound that has a number of industrial uses, including the production of laminates, glues, dinnerware, adhesives, molding compounds, coatings and flame retardants. Melamine is a name used both for the chemical and for the plastic made from it. In this event, all references are to the chemical. There are no approved direct food uses for melamine, nor are there any recommendations in the Codex Alimentarius. Melamine is illegally added to inflate the apparent protein content of food products. Because it is high in nitrogen, the addition of melamine to a food artificially increases the apparent protein content as measured with standard tests.

In this event, contamination appears to have happened as fraudulent contamination in primary production. Chinese government officials have pinpointed milk collecting stations as the sites where the melamine was added. According to Sanlu, contaminated milk was used in the manufacture of powdered infant formula processed before 6 August 2008 and the tainted milk powder has also been used in the manufacture of a number of other products..

This melamine ELISA Kit is based on indirect competitive ELISA, the coupled antigen was pre-coated in the microwells, the melamine will compete for the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of melamine contained in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of melamine in the sample.

Key facts of the Melamine ELISA Kit

• Ready to use ELISA kit based on Competitive Immunoassay
• Total time required only 75min
• Highly specific to melamine.
• Applicable for milk sample

Melamine ELISA Kit parameters

• Sensitivity: 5 ppb, ng/ml
• Stability: 12 months when stored properly
• Accuracy: 80%-120%

Melamine ELISA Kit components

• Precoated microwell, 96wells
• Melamine standard series, 0.2 - 16.2 ng/ml
• Enzyme conjugate
• TMB Substrate
• Stop solution (not available when shipping by air)
• Sample buffer
• Wash buffer

For more information about the Melamine ELISA kit, please feel free to contact us.

Further readings on melamine and melamine testing:

• World Health Organization, WHO, Chemical Risks, Melamine page
• Buka, Irena, Alvaro Osornio-Vargas, and Catherine Karr. "Melamine food contamination: Relevance to Canadian children." Paediatrics & child health 14.4 (2009): 222-224.
• FAO website, food safety and quality, Melamine.