Macrolides are a class of antibiotics derived from Saccharopolyspora erythraea (originally called Streptomyces erythreus), a type of soil-borne bacteria. Macrolides inhibit protein synthesis in bacteria by reversibly binding to the P site of the 50S unit of the ribosome. Due to the potential hazard to humans, strict MRLs have been set in EU, USA and other countries. This product utilizes the high affinity of monoclonal antibody against tylosin & tilmicosin, which can identify these two drugs. The detection limit of the Meat Macrolides Rapid Test Kit can meet both European and USA MRLs when using properly.
Key facts of the Meat Macrolides Rapid Test Kit
- Ready to use kits for lab staff
- No organic solvent required
- Result visible in 10min
- Broad-specific to all TC
Detection Limit of the Meat Macrolides Rapid Test Kit
According to EU legislation, the maximum residue limit for tylosin is 100ug/kg in all animal tissues, the MRL for tilmicosin in poultry muscle and fat is 75ug/kg, in poultry liver is 1000ug/kg, in poultry kidney is 250ug/kg.
| Drugs | MRL(ug/kg) | LOD (ug/kg) |
| Tylosin |
Muscle 100 liver 100 kidney 100 |
10 - 15 |
| Tilmicosin |
Poultry:
Other species:
|
15 - 20 |
The detection limit of this kit for tylosin is 10-90ppb, this detection limit may change, please refer to the kit insert.
Kit Components
- Macrolides Test Strip, 48pcs
- Microwell reagent, 48wells
- Plastic Pipette, 48pcs
- Sample buffer, 15ml, 2pcs
Materials required but not provided
- Micropipette, 20-200ul
- Centrifuge
- Homogenizer
Sample preparation
Meat samples shall be homogenized before testing. Basically, 2g of homogenized meat dissolved with 2ml of diluted sample buffer, shake thoroughly for 2min and then centrifuge for 10min. Take the supernate for rapid testing.
This kit can also be used in milk samples. Please check this link for more details.
