African Swine Fever Antibody Blocking ELISA, ASFV Ab Blocking ELISA Kit is an immunoassay kit to detect ASFV Ab in serum. Result in 85min. Sensitivity 95%, Specificity 100%, GMP certified.
African swine fever (ASF) is a severe viral disease affecting domestic and wild pigs;More recently (since 2007) the disease has been reported in multiple countries across Africa, Asia and Europe, in both domestic and wild pigs. In 2018 and 2019, ASFV is spreading quickly to Asia, Southeast Asia. In countries like China, Vietnam, etc now ASFV are becoming big threat to local animal farming.
This ASFV antibody ELISA kit is based on blocking ELISA to detect P30 protein specific antibodies of ASFV in swine serum / plasma, etc. which only takes 75min to get a visible result. No special instrument required and no extra cost added, which is quite suitable for lab test and field test.
The importance of antibody testing in African swine fever control
In the recent closed Anual Veterinary Diagnostic Conference in Nanjing, Dr. Luis Gimenez Lirola from Iowa State University had a great report on the testing strategies in ASF. prevention and control.
He mentioned that in acute infection of ASF, PCR positive and antibody-negative results were 66%, PCR positive and antibody-positive results were 34%, PCR negative and antibody-positive results were 0%. In subacute infection of ASF, PCR positive and antibody-negative results were 10%, PCR positive and antibody-positive results were 87%, PCR negative and antibody-positive results were 3%. In chronic infection of ASF, PCR positive and antibody-negative results were 2%, PCR positive and antibody-positive were 31%, while PCR negative and antibody-positive results were 67%.
From the research, we can find that in subacute and chronic infection of ASF, PCR negative results do not rule out ASF infection, antibody testing shall still be considered as an important tool for ASF eradication.
Key facts of ASFV Ab Blocking ELISA Kit
- Ready to use kits for farmers and general labstaff.
- No special instrument required.
- Suitable for lab large sample test.
- Result in 60min.
Technical Information for the ASFV Ab Blocking ELISA Kit
| Product Code | ZE013 |
|---|---|
| Package Size | 2*96T, 5*96T |
| Shelf-life | 12 months |
| Storage | 2-8 degree celsius at cool dark place |
| Delivery | Room temperature |
| Senstivity | >95% |
| Specificity | >99% |
| Reproducibility | Inter-lot C.V. <10%; Intra-lot C.V. <5% |
| Total time cost | 85min |
| ELISA reading | 450nm with ELISA reader |
| Approval status | Approved by China MOA |
ASFV Ab Blocking ELISA Kit Components
- Positive Control
- Negative Control
- HRP-antibody Conjugate
- Sample buffer Solution
- TMB Substrate
- Stop Solution
- Precoated 96well microtiter plate
Validation or Approval
- This ASFV Antibody Blocking ELISA Kit is approved by Chinese Ministry of Agriculture and Rural Affairs.
